pSTAP-seq_fly-ctrl
(Plasmid #86380)

• Purpose: (Empty Backbone) Vector to measure the basal activity of CP candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.
• Depositing Lab: Alexander Stark
• Publication: Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.1038/nbt.3739.

Backbone

• Vector backbone: pGL3
• Backbone manufacturer: Promega
• Backbone size (bp): 3477
• Vector type: STAP-seq screening vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

to consruct the STAP-seq screening vector from pGL3 Promoter we replaced the sequence between the BglII and FseI sites with the following sequence, containing a ccdB suicide gene next to the Chloramphenicole resistance gene flanked by homology arms (used for cloning the candidates during library generation), an intron (mhc16), an ORF (truncated sgGFP, Qbiogene, Inc)