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Addgene

Integrin alpha L - mCFP
(Plasmid #8637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Integrin, alpha L
  • Alt name
    CD11A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • GenBank ID
    NM_002209
  • Entrez Gene
    ITGAL (a.k.a. CD11A, LFA-1, LFA1A)
  • Tag / Fusion Protein
    • CFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note Addgene NGS result identified 3 mutations compared to integrin alpha-L isoform a precursor [Homo sapiens] NP_002200.2: R214W, N770S, P1136S.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Integrin alpha L - mCFP was a gift from Timothy Springer (Addgene plasmid # 8637 ; http://n2t.net/addgene:8637 ; RRID:Addgene_8637)

  • For your References section:

    Bidirectional transmembrane signaling by cytoplasmic domain separation in integrins. Kim M, Carman CV, Springer TA. Science 2003 Sep 19;301(5640):1720-5. 10.1126/science.1084174 PubMed 14500982
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