Human ICAM-1
(Plasmid #8632)

• Depositing Lab: Timothy Springer
• Publication: Staunton et al Cell 1988 Mar 25;52(6):925-33.

Backbone

• Vector backbone: pCDM8
• Backbone manufacturer: BCCM/LMBP
• Backbone size w/o insert (bp): 4356
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MC1061/P3
• Growth instructions: The host strain is MC1061/P3 and the bacteria should be cultured in 12.5ug/mL ampicillin and 16ug/mL tetracycline. (supF)
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ICAM-1
• Alt name: CD54
• Alt name: pCD1.8
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• GenBank ID: NM_000201.1
• Entrez Gene: ICAM1 (a.k.a. BB2, CD54, P3.58)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This clone will direct the expression of functional human ICAM-1 in a variety of cell types. This sequence has been deposited in Genbank with accession number J03132. Also see refs: Nature 329, 840-842 (1987) and Nature 339, 61-64 (1989)

Please use either GenBank IDs NM_000201.1 or J03132.1 as a refence sequence for the ICAM-1 insert in this plasmid. GenBank ID NM_000201.2 (updated after this plasmid was created) is not accurate for comparison.