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Human MAC-1 alpha
(Plasmid #8631)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8631 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDM8
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4356
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Tetracycline, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    MC1061/P3
  • Growth instructions
    The host strain is MC1061/P3 and the bacteria should be cultured in 12.5ug/mL ampicillin and 16ug/mL tetracycline. (supF)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAC-1 alpha
  • Alt name
    pCDM1
  • Alt name
    CD11b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4200
  • GenBank ID
    BC096348
  • Entrez Gene
    ITGAM (a.k.a. CD11B, CR3A, MAC-1, MAC1A, MO1A, SLEB6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BstXI (not destroyed)
  • 3′ cloning site BstXI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This clone will direct the expression of functional Mac-1 upon cotransfection with the beta2 subunit in cos cells. This sequence has been deposited in Genbank with accession number J03925. Also see refs: Nature 329, 840-842 (1987) and J of Immunology 146, 648-655 (1991).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human MAC-1 alpha was a gift from Timothy Springer (Addgene plasmid # 8631 ; http://n2t.net/addgene:8631 ; RRID:Addgene_8631)

  • For your References section:

    The human leukocyte adhesion glycoprotein Mac-1 (complement receptor type 3, CD11b) alpha subunit. Cloning, primary structure, and relation to the integrins, von Willebrand factor and factor B. Corbi AL, Kishimoto TK, Miller LJ, Springer TA. J Biol Chem 1988 Sep 5;263(25):12403-11. PubMed 2457584
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