pENTR223-PLXNB2
(Plasmid
#86236)
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PurposePLXNB2 full-length human cDNA CDS in gateway entry plasmid
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86236 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR223.1 with stop codon
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Backbone manufacturerNIH Mammalian Gene Collection project
- Backbone size w/o insert (bp) 2817
- Total vector size (bp) 8334
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePLXNB2
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Alt namePlexin-B2
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SpeciesH. sapiens (human)
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Insert Size (bp)5517
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Entrez GenePLXNB2 (a.k.a. MM1, Nbla00445, PLEXB2, dJ402G11.3)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis plasmid was generated by the NIH Mammalian Gene Collection project. Genbank entry: Synthetic construct Homo sapiens clone IMAGE:100069134, MGC:199145 plexin B2 (PLXNB2) mRNA, encodes complete protein GenBank: BC172440.1 It was obtained in our laboratory from the PlasmID Repository of the DNA Resource Core at Harvard Medical School.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR223-PLXNB2 was a gift from Roland Friedel (Addgene plasmid # 86236 ; http://n2t.net/addgene:86236 ; RRID:Addgene_86236) -
For your References section:
Plexin-B2 facilitates glioblastoma infiltration by modulating cell biomechanics. Huang Y, Tejero R, Lee VK, Brusco C, Hannah T, Bertucci TB, Junqueira Alves C, Katsyv I, Kluge M, Foty R, Zhang B, Friedel CC, Dai G, Zou H, Friedel RH. Commun Biol. 2021 Jan 29;4(1):145. doi: 10.1038/s42003-021-01667-4. 10.1038/s42003-021-01667-4 PubMed 33514835