pPonA-BI-Gl NORM-LacZA TER- LacZB
(Plasmid
#86212)
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Purposebeta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 86212 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPonA
- Total vector size (bp) 8020
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Vector typeMammalian Expression ; Ponasterone A inducible promoter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site NheI (unknown if destroyed)
- 5′ sequencing primer CTCAGACACGAGCTCGGTACC
- 3′ sequencing primer CGATGCGGCCGCGCTAGC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPonA-BI-Gl NORM-LacZA TER- LacZB was a gift from Robert Singer (Addgene plasmid # 86212 ; http://n2t.net/addgene:86212 ; RRID:Addgene_86212) -
For your References section:
Temporal and spatial characterization of nonsense-mediated mRNA decay. Trcek T, Sato H, Singer RH, Maquat LE. Genes Dev. 2013 Mar 1;27(5):541-51. doi: 10.1101/gad.209635.112. Epub 2013 Feb 21. 10.1101/gad.209635.112 PubMed 23431032
Map uploaded by the depositor.
