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Addgene

pYPQ220 (AsCpf1)
(Plasmid #86208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86208 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pYPQ167
  • Vector type
    Plant Expression ; Gateway compatible Cpf1 vectors

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AsCpf1
  • Alt name
    AsCas12a
  • Species
    Acidaminococcus sp. BV3L6
  • Tag / Fusion Protein
    • 5' and 3' NLS

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI-HindIII, (unknown if destroyed)
  • 3′ cloning site MfeI-BamHI (unknown if destroyed)
  • 5′ sequencing primer gtaaaacgacggccag
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYPQ220 (AsCpf1) was a gift from Yiping Qi (Addgene plasmid # 86208 ; http://n2t.net/addgene:86208 ; RRID:Addgene_86208)

  • For your References section:

    A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z, Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18. 10.1038/nplants.2017.18 PubMed 28211909
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