pYPQ203 (pMDC32-Ubi1)
(Plasmid #86207)

• Purpose: (Empty Backbone) Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and Hygromiycin resistance marker
• Depositing Lab: Yiping Qi
• Publication: Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.

Backbone

• Vector backbone: pMDC32
• Modifications to backbone: Arabidopsis ubiquitin 10 (AtUbi10) promoter added to pYPQ202 backbone
• Vector type: Plant Expression ; Gateway compatible attR1-attR2 destination vector
• Promoter: Maize ubiquitin 1 (ZmUbi1)
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • pYPQ203 (pMDC32-Ubi1).gbk
• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: Addgene's quality control sequencing finds a 1.2kb transposable element upstream of the Kanamycin resistance cassette. This inserted sequence does not affect plasmid function.