pStI6
(Plasmid
#8616)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 8616 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET-28a
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5252
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameRibonuclease inhibitor StI
-
SpeciesSaccharopolyspora erythraea, Strain NRRL 2338
-
Insert Size (bp)365
-
MutationS2G point mutation; 19 silent mutations improve production in E. coli.
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Production plasmid for ribonuclease inhibitor and barstar
homolog StI from Saccharopolyspora erythraea. Structural gene for inhibitor StI from Saccharopolyspora
erythraea strain NRRL 2338. N-terminal serine of wild type
changed to glycine and 19 silent mutations improve production
in E. coli.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pStI6 was a gift from Robert Hartley (Addgene plasmid # 8616 ; http://n2t.net/addgene:8616 ; RRID:Addgene_8616) -
For your References section:
A new member of the bacterial ribonuclease inhibitor family from Saccharopolyspora erythraea. Krajcikova D, Hartley RW. FEBS Lett 2004 Jan 16;557(1-3):164-8. 10.1016/S0014-5793(03)01468-6 PubMed 14741361