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Addgene

pCali2
(Plasmid #86119)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETDuet-1
  • Backbone manufacturer
    Novagen
  • Modifications to backbone
    Replaced pETDuet-1 T7 promoters and ORFs with: pTlpA and pLacI pR/pL and pLacI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    TlpA36
  • Species
    S. Typhimurium
  • Insert Size (bp)
    1116
  • Mutation
    D341A, P60L, D135V, K187R, K202I, L208Q
  • GenBank ID
    M88208.1
  • Promoter pTlpA, pLacI

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACCGTTTACGAGATC
  • 3′ sequencing primer gcctcactgattaagcattgg
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    mWasabi
  • Species
    Synthetic
  • Insert Size (bp)
    756
  • GenBank ID
    EU024648.1
  • Promoter pTlpA
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATGCGTCCGGCGTAGA
  • 3′ sequencing primer gccagccctgcttcaata
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    TcI
  • Alt name
    cI842
  • Alt name
    Lambda Repressor
  • Insert Size (bp)
    714
  • Mutation
    A67T
  • Promoter pR/pL, pLacI

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer aagttggacatcacctcc
  • 3′ sequencing primer ACTACTGGGCTGCTTCC
    • (Common Sequencing Primers)

Gene/Insert 4

  • Gene/Insert name
    mCherry
  • Promoter pR/pL

Cloning Information for Gene/Insert 4

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACTGAGCACATCAGCAGG
  • 3′ sequencing primer gtgttaatGGTTTCTTTTTTGTGCTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCali2 was a gift from Mikhail Shapiro (Addgene plasmid # 86119 ; http://n2t.net/addgene:86119 ; RRID:Addgene_86119)

  • For your References section:

    Tunable thermal bioswitches for in vivo control of microbial therapeutics. Piraner DI, Abedi MH, Moser BA, Lee-Gosselin A, Shapiro MG. Nat Chem Biol. 2017 Jan;13(1):75-80. doi: 10.1038/nchembio.2233. Epub 2016 Nov 14. 10.1038/nchembio.2233 PubMed 27842069
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