pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS
(Plasmid #85821)

• Purpose: iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
• Depositing Lab: Joachim Wittbrodt
• Publication: Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261.

Backbone

• Vector backbone: pDestTol2pA2
• Backbone manufacturer: Chi-Bin Chen
• Backbone size w/o insert (bp): 6141
• Total vector size (bp): 11582
• Vector type: Tol2 based vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Zf Ubi::cMyc oDam_f_GFPoNLS
• Alt name: Ubi::cMyc oDamGFP
• Species: Ubi (Ubiquitin-B promoter): Danio rerio (Zebrafish); Dam: E. coli;. GFP: Aequorea victoria
• Insert Size (bp): 5441
• Mutation: L122A. Codon optimization compatible to work in Medaka and Zebrafish (other species not tested). Cryptic splicing sites removed.
• Tags / Fusion Proteins:
  • cMyc N terminal to oDam_f_GFPoNLS
  • oNLS (optimized nuclear localization signal) C terminal to the GFP

Resource Information

• Supplemental Documents:
  • pDestTol Ubi cMyc_oDamFGoN cmlc2EGFP.gb
  • pDestTol ZfUbi cMyc oDam_f_GFPoNLS Addgene.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry