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Addgene

pKI1.1
(Plasmid #85807)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85807 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR/D-TOPO
  • Total vector size (bp) 9326
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human-codon-optimized SpCas9
  • Species
    Streptococcus pyogenes
  • Promoter AtRPS5A
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • NLS (N terminal on insert)
    • NLS (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    human-codon-optimized SpCas9 was closed from pX330-U6-Chimeric_BB-CBh-hSpCas9 (item #42230) that we bought from Addgene on 6th June, 2013 by Prof. Tetsuya Higashiyama

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKI1.1 was a gift from Tetsuya Higashiyama (Addgene plasmid # 85807 ; http://n2t.net/addgene:85807 ; RRID:Addgene_85807)

  • For your References section:

    pKAMA-ITACHI vectors for highly efficient CRISPR/Cas9-mediated gene knockout in Arabidopsis thaliana. Tsutsui H, Higashiyama T. Plant Cell Physiol. 2016 Nov 17. pii: pcw191. 10.1093/pcp/pcw191 PubMed 27856772
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