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Purpose(Empty Backbone) Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85745 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLentiGuide
- Backbone size (bp) 9127
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Vector typeLentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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A portion of this plasmid was derived from a plasmid made byLentiGuide (Feng Zhang)
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Lenti-entry-puro was a gift from Qin Yan (Addgene plasmid # 85745 ; http://n2t.net/addgene:85745 ; RRID:Addgene_85745) -
For your References section:
An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting. Cao J, Wu L, Zhang SM, Lu M, Cheung WK, Cai W, Gale M, Xu Q, Yan Q. Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25. 10.1093/nar/gkw660 PubMed 27458201
