pEGFP-PLAA
(Plasmid #85669)

• Purpose: Expression of PLAA with N-terminal GFP tag
• Depositing Lab: Hemmo Meyer
• Publication: Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Backbone

• Vector backbone: pEGFP-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PLAA
• Alt name: Phospholipase A2-activating protein
• Alt name: DOA1, PLA2P, PLAP
• Species: H. sapiens (human)
• GenBank ID: BC032551.1
• Entrez Gene: PLAA (a.k.a. DOA1, NDMSBA, PLA2P, PLAP)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: cloned from Image clone IRATp970D0156D from Source Bioscience
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry