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Addgene

pEGFP-YOD1-MutS2
(Plasmid #85667)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85667 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YOD1
  • Alt name
    YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
  • Alt name
    DKFZp451J1719, OTUD2, PRO0907
  • Species
    H. sapiens (human)
  • Mutation
    I292Q and V295Q
  • GenBank ID
    NM_018566
  • Entrez Gene
    YOD1 (a.k.a. DUBA8, OTUD2, PRO0907)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

site-directed mutagenesis on pEGFP-YOD1 (#85664)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-YOD1-MutS2 was a gift from Hemmo Meyer (Addgene plasmid # 85667 ; http://n2t.net/addgene:85667 ; RRID:Addgene_85667)

  • For your References section:

    VCP/p97 cooperates with YOD1, UBXD1 and PLAA to drive clearance of ruptured lysosomes by autophagy. Papadopoulos C, Kirchner P, Bug M, Grum D, Koerver L, Schulze N, Poehler R, Dressler A, Fengler S, Arhzaouy K, Lux V, Ehrmann M, Weihl CC, Meyer H. EMBO J. 2016 Oct 17. pii: e201695148. 10.15252/embj.201695148 PubMed 27753622
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