mCherry-YOD1
(Plasmid #85663)

• Purpose: Expression of human YOD1 with N-terminal mCherry tag
• Depositing Lab: Hemmo Meyer
• Publication: Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Backbone

• Vector backbone: pmCherry-C1
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YOD1
• Alt name: YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
• Alt name: DKFZp451J1719, OTUD2, PRO0907
• Species: H. sapiens (human)
• GenBank ID: NM_018566
• Entrez Gene: YOD1 (a.k.a. DUBA8, OTUD2, PRO0907)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

cloned from pEGFP-YOD1 (#85664)