pKTol2P-PTK
(Plasmid
#85599)
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PurposeTol2 transposon with a puromycin-thymidine kinase fusion driven by a PGK promoter.
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85599 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepK
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Backbone manufacturerK. Clark
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTol2 transposon with a puromycin-thymidine kinase fusion
- Promoter PGK
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer mPGK-F (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKTol2P-PTK was a gift from Karl Clark & Scott Fahrenkrug (Addgene plasmid # 85599 ; http://n2t.net/addgene:85599 ; RRID:Addgene_85599) -
For your References section:
Enzymatic engineering of the porcine genome with transposons and recombinases. Clark KJ, Carlson DF, Foster LK, Kong BW, Foster DN, Fahrenkrug SC. BMC Biotechnol. 2007 Jul 17;7:42. 10.1186/1472-6750-7-42 PubMed 17640337
