pBad-sfGFP
(Plasmid #85482)

• Purpose: Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag
• Depositing Lab: Ryan Mehl
• Publication: Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77. doi: 10.1021/bi901947r.

Backbone

• Vector backbone: pBAD/Myc-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 4900
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: superfolder GFP
• Alt name: sfGFP
• Species: Synthetic
• Insert Size (bp): 744
• Promoter: Arabinose
• Tag / Fusion Protein:
  • 6x His (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pBad forward
• 3′ sequencing primer: pBad reverse

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry