pGST-PSP-hTG
(Plasmid
#85470)
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Purposebacterial expression of human tissue transglutaminase
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX
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Modifications to backbonea PreScission Protease site (PSP) was introduced by a site-overlap extension mutation strategy
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namehTG2
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Alt nametransglutaminase 2
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SpeciesH. sapiens (human)
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Entrez GeneTGM2 (a.k.a. G(h), TG(C), TGC, hTG2, tTG)
- Promoter tac
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Tags
/ Fusion Proteins
- GST (N terminal on backbone)
- PSP (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pGEX-5'
- 3′ sequencing primer pGEX-3' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGST-PSP-hTG was a gift from Jeffrey Keillor & Ray Truant (Addgene plasmid # 85470 ; http://n2t.net/addgene:85470 ; RRID:Addgene_85470) -
For your References section:
Expression, purification and kinetic characterisation of human tissue transglutaminase. Roy I, Smith O, Clouthier CM, Keillor JW. Protein Expr Purif. 2013 Jan;87(1):41-6. doi: 10.1016/j.pep.2012.10.002. Epub 2012 Oct 22. 10.1016/j.pep.2012.10.002 PubMed 23085087
