pAAV-RSV-SpCas9
(Plasmid #85450)

• Purpose: Express SpCas9 in mammalian cells
• Depositing Lab: Hetian Lei
• Publication: Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.729467.

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Backbone

• Vector backbone: pAAV
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pRSV
• Promoter: pRSV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CGGTCTAGAaatgtagtcttatgcaatac
• 3′ sequencing primer: CGGACCGGTTTTATGTATCGAGCTAGGCAC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We purchased a lentiviral vector with shRNA-MDM2 from GE Dharmacon (Lafayette, CO)
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We replaced the pMecp2 promoter of SpCas9 (Addgene: 60957) with a RSV (Rous Sarcoma Virus) promoter, which was amplified from a lentiviral vector for shRNA-MDM2