HOXB9 (murine) HIS-tag pET
(Plasmid
#8541)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8541 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET28b
- Backbone size w/o insert (bp) 5400
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXB9
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Alt nameHOX2E
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Alt nameHOX2.5
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SpeciesM. musculus (mouse)
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Insert Size (bp)1000
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MutationMissing the first 13 amino acids of HOXB9; makes protein by Western
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GenBank IDM34857
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Entrez GeneHoxb9 (a.k.a. Hox-2.5)
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Tags
/ Fusion Proteins
- T7 (N terminal on backbone)
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWilliam McGinnis
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXB9 (murine) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8541 ; http://n2t.net/addgene:8541 ; RRID:Addgene_8541) -
For your References section:
Pbx modulation of Hox homeodomain amino-terminal arms establishes different DNA-binding specificities across the Hox locus. Chang CP, Brocchieri L, Shen WF, Largman C, Cleary ML. Mol Cell Biol 1996 Apr;16(4):1734-45. PubMed 8657149
Map uploaded by the depositor.