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Addgene

HOXB9 (murine) HIS-tag pET
(Plasmid #8541)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8541 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXB9
  • Alt name
    HOX2E
  • Alt name
    HOX2.5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • Mutation
    Missing the first 13 amino acids of HOXB9; makes protein by Western
  • GenBank ID
    M34857
  • Entrez Gene
    Hoxb9 (a.k.a. Hox-2.5, MGC124051, MGC124052, MGC124053, MGC124054)
  • Tags / Fusion Proteins
    • T7 (N terminal on backbone)
    • His (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    William McGinnis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXB9 (murine) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8541 ; http://n2t.net/addgene:8541 ; RRID:Addgene_8541)

  • For your References section:

    Pbx modulation of Hox homeodomain amino-terminal arms establishes different DNA-binding specificities across the Hox locus. Chang CP, Brocchieri L, Shen WF, Largman C, Cleary ML. Mol Cell Biol 1996 Apr;16(4):1734-45. PubMed 8657149
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