Lenti-multi-CRISPR
(Plasmid #85402)

• Purpose: (Empty Backbone) Lentiviral vector for the delivery of multiple sgRNAs targeting different genes with constitutive Cas9 expression
• Depositing Lab: Qin Yan
• Publication: Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e149. Epub 2016 Jul 25.

Backbone

• Vector backbone: Lenti-CRISPR
• Backbone size (bp): 15069
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Copy number: High Copy

Resource Information

• A portion of this plasmid was derived from a plasmid made by: LentiCRISPR (Feng Zhang)
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Information for Cloning Grade DNA (Catalog # 85402-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here