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Addgene

Lenti-iCas9-neo Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb extended ladder and a digestion of the plasmid Lenti-iCas9-neo with the following enzymes: BsmBI-HF, XhoI, BamHI-HF, and NheI-HF.

Expected Bands
BsmBI: 15.2 kb
BsmBI/ XhoI: 10 kb & 5.2 kb
BamHI-HF/ NheI-HF: 7.4 kb, 4.8 kb, & 3 kb

We observe the expected bands for this digest.