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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 8527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4076
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Vector typeMammalian Expression ; In vitro transcriptioin/translation
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHOXB4
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Alt nameHOX2F
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Alt nameHOX2.6
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SpeciesH. sapiens (human)
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Insert Size (bp)2000
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GenBank IDNM_024015
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Entrez GeneHOXB4 (a.k.a. HOX-2.6, HOX2, HOX2F)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byEdoardo Boncinelli
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXB4 (human) pSG5 was a gift from Corey Largman (Addgene plasmid # 8527 ; http://n2t.net/addgene:8527 ; RRID:Addgene_8527) -
For your References section:
Lineage-restricted expression of homeobox-containing genes in human hematopoietic cell lines. Shen WF, Largman C, Lowney P, Corral JC, Detmer K, Hauser CA, Simonitch TA, Hack FM, Lawrence HJ. Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40. 10.1073/pnas.86.21.8536 PubMed 2573064
Map uploaded by the depositor.