HOXB4 (human) HA-TAT-tag pET
(Plasmid #8526)

• Depositing Lab: Corey Largman
• Publication: Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21):8536-40.

Backbone

• Vector backbone: pET28
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXB4
• Alt name: HOX2F
• Alt name: HOX2.6
• Species: H. sapiens (human)
• Insert Size (bp): 959
• Mutation: replace T7 and HIS tags of pET with an HA tag;
• GenBank ID: NM_024015
• Entrez Gene: HOXB4 (a.k.a. HOX-2.6, HOX2, HOX2F)
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Edoardo Boncinelli

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry