pDG279
(Plasmid #85219)

• Purpose: mammalian expression of ubiquitin-K48R-EGFP fusion
• Depositing Lab: Douglas Gray
• Publication: Tsirigotis et al J Biol Chem. 2001 Dec 7;276(49):46073-8.

Backbone

• Vector backbone: unknown
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ubiquitin C
• Species: H. sapiens (human)
• Mutation: K48R
• Entrez Gene: UBC (a.k.a. HMG20)
• Promoter: human ubiquitin C
• Tags / Fusion Proteins:
  • 6xHIS (N terminal on insert)
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: hUBCpro-F
• 3′ sequencing primer: EGFP-N

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry