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Addgene

pCAG-mCherry-FKBP-GEF(ARHGEF1)
(Plasmid #85152)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85152 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGEN
  • Backbone manufacturer
    Addgene plasmid #11160
  • Backbone size w/o insert (bp) 4798
  • Total vector size (bp) 6661
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fusion of mCherry, FKBP, and the DH-domain of ARHGEF1 (amino acids 380-630)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1875
  • Entrez Gene
    ARHGEF1 (a.k.a. GEF1, IMD62, LBCL2, LSC, P115-RHOGEF, SUB1.5)
  • Promoter CAG
  • Tag / Fusion Protein
    • mCherry-FKBP (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTTATTGTGCTGTCTCATC
  • 3′ sequencing primer ACACCAGCCACCACCTTC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For synthetic activation of Rho through Rapamycin-induced translocation of a RhoGEF domain to the plasma membrane. Requires co-expression with plasma membrane-localized FRB, e.g. Lyn11-targeted FRB, Addgene plasmid #20147

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-mCherry-FKBP-GEF(ARHGEF1) was a gift from Tobias Meyer (Addgene plasmid # 85152 ; http://n2t.net/addgene:85152 ; RRID:Addgene_85152)

  • For your References section:

    Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, Betzig E, Meyer T. Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub 2016 Nov 14. 10.1038/ncb3438 PubMed 27842057
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