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Addgene

pLV-CDH5-mRuby3-IRES-puro
(Plasmid #85144)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85144 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLV-CAG-IRES-Puro
  • Backbone size w/o insert (bp) 8785
  • Total vector size (bp) 12398
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CDH5-mRuby3 (VE-cadherin, C-terminally tagged with mRuby3
  • Alt name
    CDH5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3114
  • Entrez Gene
    CDH5 (a.k.a. 7B4, CD144)
  • Promoter CAG
  • Tag / Fusion Protein
    • mRuby3 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GTTATTGTGCTGTCTCATC
  • 3′ sequencing primer ACACCGGCCTTATTCCAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Remark on cloning and propagating of cDNA encoding human CDH5: In our experience, CDH5 cDNA was toxic to bacteria when cloned into CMV-driven expression plasmids, resulting in small or mutated clones.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-CDH5-mRuby3-IRES-puro was a gift from Tobias Meyer (Addgene plasmid # 85144 ; http://n2t.net/addgene:85144 ; RRID:Addgene_85144)

  • For your References section:

    Engulfed cadherin fingers are polarized junctional structures between collectively migrating endothelial cells. Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, Betzig E, Meyer T. Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub 2016 Nov 14. 10.1038/ncb3438 PubMed 27842057
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