pCAG-AMPH1-BAR-mCherry
(Plasmid #85130)

• Purpose: BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry
• Depositing Lab: Tobias Meyer
• Publication: Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub 2016 Nov 14.

Backbone

• Vector backbone: pCAGEN
• Backbone size w/o insert (bp): 4798
• Total vector size (bp): 6292
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: BAR domain of human Amphiphysin1 (amino acids 1-256), C-terminally tagged with mCherry
• Alt name: AMPH
• Species: H. sapiens (human)
• Insert Size (bp): 1494
• GenBank ID: NM_001635
• Entrez Gene: AMPH (a.k.a. AMPH1)
• Promoter: CAG
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: GTTATTGTGCTGTCTCATC
• 3′ sequencing primer: ACACCAGCCACCACCTTC

Resource Information

• Supplemental Documents:
  • P882_AMPH-BAR-mCherry.gb
• A portion of this plasmid was derived from a plasmid made by: ORFeome v5.1
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Compatible with 2nd and 3rd generation lentiviral packaging systems.