pKSCTMF
(Plasmid #85073)

• Purpose: Gene insertion/deletion in Pichia pastoris
• Depositing Lab: Sheng Yang
• Publication: Yang et al FEMS Yeast Res. 2009 Jun;9(4):600-9. doi: 10.1111/j.1567-1364.2009.00503.x. Epub 2009 Mar 25.

Backbone

• Vector backbone: pBluescript II KS(-)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 6000
• Vector type: Gene insertion/deletion in Pichia pastoris
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AOX1promoter - mazF - AOX1 tt- Zeocin R gene
• Species: E. coli
• Insert Size (bp): 3000
• GenBank ID: mazF NP_417262.1
• Promoter: pAOX1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (unknown if destroyed)
• 3′ cloning site: SmaI (unknown if destroyed)
• 5′ sequencing primer: T7 and 5 AOX1
• 3′ sequencing primer: T3 and 3AOX1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pDGIEF _Prof. Shun-Peng Li lab, Nanjing Agricultural University

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry