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Addgene

pUASTattb-cAMPr Y205A, R626A
(Plasmid #84932)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84932 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    puastattb
  • Backbone size w/o insert (bp) 8000
  • Total vector size (bp) 10700
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cAMPr Y205A R626A
  • Species
    Synthetic
  • Insert Size (bp)
    2300
  • Mutation
    PKA-C Y205A, PKA-R R626A
  • Promoter 5X UAS

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AGGGAATTGGGAATTCAAAATGGGCAACGCCGCCGCCGCCAAGAAG
  • 3′ sequencing primer ATCTGTTAACGAATTTTACATCTTCCGCTTTCTCAGCGTGCTTC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASTattb-cAMPr Y205A, R626A was a gift from Justin Blau (Addgene plasmid # 84932 ; http://n2t.net/addgene:84932 ; RRID:Addgene_84932)

  • For your References section:

    cAMPr: A single-wavelength fluorescent sensor for cyclic AMP. Hackley CR, Mazzoni EO, Blau J. Sci Signal. 2018 Mar 6;11(520). pii: 11/520/eaah3738. doi: 10.1126/scisignal.aah3738. 10.1126/scisignal.aah3738 PubMed 29511120
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