pCAG-Cas9-162A
(Plasmid #84918)

• Purpose: for in vitro transcription of Cas9 with polyA tail encoded
• Depositing Lab: Ralf Kuehn
• Publication: Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.

Backbone

• Vector backbone: pCAG
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cas9
• Species: S. pyogenes
• Promoter: CAG
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • Flag (N terminal on insert)
  • NLS (C terminal on insert)
  • polyA tail (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7
• 3′ sequencing primer: M13F20

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry