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pAav-MCS-PQS2-3xHA
(Plasmid #84917)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84917 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-MCS
  • Backbone manufacturer
    agilent
  • Backbone size w/o insert (bp) 4650
  • Total vector size (bp) 4890
  • Vector type
    AAV
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LOX-PGK-NEO-LOX
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1989
  • Promoter mPGK
  • Tag / Fusion Protein
    • PQS2 3xHA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer CCTCTGACTTGAGCGTCGAT
  • 3′ sequencing primer TACTATGGTTGCTTTGACGT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Sequencing over ITR sequences may be difficult

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAav-MCS-PQS2-3xHA was a gift from Sven Eyckerman (Addgene plasmid # 84917 ; http://n2t.net/addgene:84917 ; RRID:Addgene_84917)

  • For your References section:

    An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics. Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S. Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220. 10.1038/srep27220 PubMed 27264994
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