pEGFP-N1-ACTR3
(Plasmid #8462)

• Depositing Lab: Matthew Welch
• Publication: Welch et al J Cell Biol 1997 Jul 28;138(2):375-84.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: BD Biosciences Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ACTR3
• Alt name: ARP3
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• GenBank ID: AF006083
• Entrez Gene: ACTR3 (a.k.a. ARP3)
• Tag / Fusion Protein:
  • GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry