PPRE-pNL1.3
(Plasmid #84394)

• Purpose: Secreted Luciferase reporter for PPARgamma activity
• Depositing Labs: Severine Degrelle, Thierry Fournier
• Publication: Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 Nov 1.

Backbone

• Vector backbone: pNL1.3[secNluc]
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3200
• Total vector size (bp): 4600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPARG response element
• Alt name: PPRE
• Alt name: ApoCIII promotor
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• Entrez Gene: PPARG (a.k.a. CIMT1, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma)
• Promoter: PPRE
• Tag / Fusion Protein:
  • secNluc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: RVprimer3

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry