pAd-Track Flag-SIRT1 H355A
(Plasmid #8439)

• Depositing Lab: Pere Puigserver
• Publication: Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Backbone

• Vector backbone: pAd-Track-CMV
• Backbone size w/o insert (bp): 9220
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SIRT1
• Species: M. musculus (mouse)
• Insert Size (bp): 2300
• Mutation: Histidine 355 mutated to Alanine
• Entrez Gene: Sirt1 (a.k.a. SIR2L1, Sir2, Sir2a, Sir2alpha)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: No

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry