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Purpose(Empty Backbone) E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 84301 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepYES1L
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Backbone manufacturerInvitrogen
- Backbone size (bp) 10473
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Modifications to backboneRestriction sites BamH1, EcoR1 were added at the assembly site, URA3 gene and promoter were incorporated as optional counter selection for assembly.
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersTRP1, URA3
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pYES1L-URA was a gift from George Church (Addgene plasmid # 84301 ; http://n2t.net/addgene:84301 ; RRID:Addgene_84301) -
For your References section:
Design, synthesis, and testing toward a 57-codon genome. Ostrov N, Landon M, Guell M, Kuznetsov G, Teramoto J, Cervantes N, Zhou M, Singh K, Napolitano MG, Moosburner M, Shrock E, Pruitt BW, Conway N, Goodman DB, Gardner CL, Tyree G, Gonzales A, Wanner BL, Norville JE, Lajoie MJ, Church GM. Science. 2016 Aug 19;353(6301):819-22. doi: 10.1126/science.aaf3639. 10.1126/science.aaf3639 PubMed 27540174
Map uploaded by the depositor.
