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Addgene

pMyCA
(Plasmid #84268)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 84268 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAL5000, pUC19
  • Backbone size (bp) 5994
  • Modifications to backbone
    Insertion of the gene AmiC and AmiA of M. smegmatis
  • Vector type
    Bacterial Expression
  • Promoter Aceteamidase
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    it is a shuttle vector, to be used in Mycobacterium smegmatis
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMyCA was a gift from Young-Hwa Song (Addgene plasmid # 84268 ; http://n2t.net/addgene:84268 ; RRID:Addgene_84268)

  • For your References section:

    A versatile vector for mycobacterial protein production with a functional minimized acetamidase regulon. Magana Vergara C, Kallenberg CJL, Rogasch M, Hubner CG, Song YH. Protein Sci. 2017 Aug 31. doi: 10.1002/pro.3288. 10.1002/pro.3288 PubMed 28857325
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