pHD157
(Plasmid #84033)

• Purpose: Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the fabp10 promoter and Venus under the crystalin promoter in zebrafish
• Depositing Labs: Daniel Hesselson, Didier Stainier
• Publication: Ni et al Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15389-94. Epub 2012 Aug 20.

Backbone

• Vector backbone: unknown
• Vector type: Cre/Lox ; Zebrafish transgenesis

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cre
• Promoter: fabp10
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: mCherry-F (ccccgtaatgcagaagaaga)
• 3′ sequencing primer: SV40pA-R

Gene/Insert 2

• Gene/Insert name: Venus
• Promoter: Crystalin

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: T3
• 3′ sequencing primer: BGH-rev

Resource Information

• Supplemental Documents:
  • pHD157

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Designed in a pBluescript series vector containing I-SceI meganuclease sites. The I-SceI meganuclease method is a common method for establishing transgenic zebrafish lines.