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Human Subpooled CRISPRa-v2 Libraries
(Pooled Libraries #83980, #83981, #83982, #83983, #83984, #83985, #83986)

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  • Purpose

    Version 2 - Human subpooled CRISPRa libraries for activation of gene transcription. Available as top 5 gRNAs per gene.

  • Vector Backbone

    pCRISPRia-v2 (Plasmid #84832) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 83980

  • Kinases, Phosphatases, and Drug Targets - gRNA pooled library †

1 $430 Add to Cart
Pooled Library 83981

  • Cancer and Apoptosis - gRNA pooled library †

1 $430 Add to Cart
Pooled Library 83982

  • Stress and Proteostasis - gRNA pooled library †

1 $325 Add to Cart
Pooled Library 83983

  • Mitochondria, Trafficking, and Motility - gRNA pooled library †

1 $325 Add to Cart
Pooled Library 83984

  • Gene expression - gRNA pooled library †

1 $430 Add to Cart
Pooled Library 83985

  • Membrane Proteins - gRNA pooled library †

1 $430 Add to Cart
Pooled Library 83986

  • Unassigned - gRNA pooled library †

1 $325 Add to Cart
Available to academic and nonprofits only.

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with SunTag-VP64 constructs (#60903 and #60904) or otherwise used with cell lines already expressing activator dCas9.

  • If you would like to receive all seven sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Library Details

Pooled Library Genes Targeted Targeting gRNAs Control gRNAs
83980 2,320 12,780 250
83981 2,921 16,055 280
83982 3,093 16,615 290
83983 2,220 12,035 250
83984 2,288 12,445 250
83985 2,405 12,895 250
83986 3,668 19,820 325
  • Species
    Human
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    12.5 ng/µL

Resource Information

Depositor Comments

In addition to SunTag-VP64 constructs (#60903 and #60904), another dCas9-containing construct that can be used with these libraries is dCas9-VPR (#63798).

The Weissman Lab's sequencing data analysis pipeline (Link opens in a new window) is available on GitHub.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83980)
    hCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83981)
    hCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83982)
    hCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83983)
    hCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83984)
    hCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83985)
    hCRISPRa-v2 subpooled library was a gift from Jonathan Weissman (Addgene #83986)

  • For your References section:

    Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation. Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS. eLife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760. PubMed 27661255
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