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PurposeContains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 83959 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR2.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3929
- Total vector size (bp) 4029
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Modifications to backboneNone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameInternal Control sequence
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Alt nameInC
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SpeciesSynthetic
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Insert Size (bp)100
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13 Reverse
- 3′ sequencing primer T7 promoter (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was created in the FDA ORA lab.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR2.1-InC was a gift from Kai-Shun Chen (Addgene plasmid # 83959 ; http://n2t.net/addgene:83959 ; RRID:Addgene_83959)
