pT7-CalfluxVTN
(Plasmid #83926)

• Purpose: Bioluminescent Reporter of Intracellular Calcium
• Depositing Lab: Carl Johnson
• Publication: Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10.1038/ncomms13268.

Backbone

• Vector backbone: pRSETb
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2787
• Total vector size (bp): 4224
• Modifications to backbone: The T7-tag, Xpress tag and Multiple Cloning Site of pRSETb was removed via restriction digestion of the NheI and HindIII sites. An EcoRI site was inserted downstream of the new NheI site, and CalfluxVTN was inserted via the new EcoRI and HindIII restriction sites.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CalfluxVTN
• Alt name: Venus-TnC-NanoLuc
• Species: Synthetic
• Insert Size (bp): 1437
• Mutation: None
• GenBank ID:
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry