PET11a-Af2sir2
(Plasmid #8383)

• Depositing Lab: Cynthia Wolberger
• Publication: Avalos et al Mol Cell 2002 Sep;10(3):523-35.

Backbone

• Vector backbone: PET11a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5677
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21(DE3)plyss
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Sir2-Af2 full length
• Alt name: NAD-dependent protein deacetylases
• Alt name: Regulatory protein SIR2 homolog 2
• Species: Archaeoglobus Fulgidus
• Insert Size (bp): 759
• Mutation: PET11a-Af2Sir2D103N
• GenBank ID: NP_068953
• Entrez Gene: SIRT2 (a.k.a. SIR2, SIR2L, SIR2L2)
• Entrez Gene: Sir2.2
• Entrez Gene: SIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry