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Addgene

pProEX-Af2Sir2
(Plasmid #8380)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8380 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pProEXHT
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4750
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Bl-21 cells
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sir2-Af2 full length
  • Alt name
    NAD-dependent protein deacetylases
  • Alt name
    Regulatory protein SIR2 homolog 2
  • Species
    Archaeoglobus Fulgidus
  • Insert Size (bp)
    759
  • GenBank ID
    NP_068953
  • Entrez Gene
    SIRT2 (a.k.a. SIR2, SIR2L, SIR2L2)
  • Entrez Gene
    Sir2.2
  • Entrez Gene
    SIRT1 (a.k.a. SIR2, SIR2L1, SIR2alpha)
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13/pUC reverse 23
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pProEX-Af2Sir2 was a gift from Cynthia Wolberger (Addgene plasmid # 8380 ; http://n2t.net/addgene:8380 ; RRID:Addgene_8380)

  • For your References section:

    Structure of a Sir2 enzyme bound to an acetylated p53 peptide. Avalos JL, Celic I, Muhammad S, Cosgrove MS, Boeke JD, Wolberger C. Mol Cell 2002 Sep;10(3):523-35. 10.1016/S1097-2765(02)00628-7 PubMed 12408821
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