Skip to main content
Addgene

pcDNA3-ProCY-N
(Plasmid #83289)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 83289 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ProCY-N negative control fluorescent sensor
  • Species
    Synthetic
  • Insert Size (bp)
    1900
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer pCMV
  • 3′ sequencing primer Sp6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-ProCY-N was a gift from Huiwang Ai (Addgene plasmid # 83289 ; http://n2t.net/addgene:83289 ; RRID:Addgene_83289)

  • For your References section:

    A Genetically Encoded FRET Sensor for Hypoxia and Prolyl Hydroxylases. Youssef S, Ren W, Ai HW. ACS Chem Biol. 2016 Jul 14. 10.1021/acschembio.6b00330 PubMed 27385075
Related items:
Commonly requested with: