pAAV-CAG-RLuc
(Plasmid #83282)

• Purpose: recombinant AAV vector packaging single-stranded Renilla Luciferase under the CAG promoter (includes WPRE element)
• Depositing Lab: Mark Kay
• Publication: Unpublished Mark Kay lab plasmids (unpublished)

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Backbone

• Vector backbone: pAAV with CAG promoter
• Backbone size w/o insert (bp): 4721
• Total vector size (bp): 5657
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: ITR integrity should be checked by performing digest with AhdI and also with XmaI
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Renilla Luciferase
• Species: Renilla reniformis
• Insert Size (bp): 936
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: GTTATTGTGCTGTCTCATC
• 3′ sequencing primer: CATTAAAGCAGCGTATCC

Resource Information

• Supplemental Documents:
  • pAAV-CAG-RLuc
• A portion of this plasmid was derived from a plasmid made by: Gene for RLuc was amplified from Promega plasmid pRL-TK, pAAV backbone as well as CAG promoter and WPRE sequence were obtained from Addgene plasmid #37825

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry