pAD-CMV-Caveolin1-CMV-GFP
(Plasmid
#83272)
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PurposeAdenoviral expression of Caveolin1 with co-expression of GFP
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 83272 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAD/CMV/V5-DEST
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Backbone manufacturerInvitrogen
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Vector typeAdenoviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert 1
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Gene/Insert nameCAV1
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Alt namecaveolin 1
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SpeciesH. sapiens (human)
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Entrez GeneCAV1 (a.k.a. BSCL3, CGL3, LCCNS, MSTP085, PPH3, VIP21)
- Promoter CMV
Cloning Information for Gene/Insert 1
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer SV40pA-R (5'-GAAATTTGTGATGCTATTGC-3') (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameeGFP
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Speciesjellyfish
- Promoter CMV
Cloning Information for Gene/Insert 2
- Cloning method Gateway Cloning
- 5′ sequencing primer LNCX (5'-AGCTCGTTTAGTGAACCGTCAGATC-3')
- 3′ sequencing primer TK-pA-R2 (5'-GGTGGGGAAAAGGAAGAAAC-3') (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAD-CMV-Caveolin1-CMV-GFP was a gift from Andrew Brooks (Addgene plasmid # 83272 ; http://n2t.net/addgene:83272 ; RRID:Addgene_83272) -
For your References section:
Caveolin-1 is necessary for hepatic oxidative lipid metabolism: evidence for crosstalk between caveolin-1 and bile acid signaling. Fernandez-Rojo MA, Gongora M, Fitzsimmons RL, Martel N, Martin SD, Nixon SJ, Brooks AJ, Ikonomopoulou MP, Martin S, Lo HP, Myers SA, Restall C, Ferguson C, Pilch PF, McGee SL, Anderson RL, Waters MJ, Hancock JF, Grimmond SM, Muscat GE, Parton RG. Cell Rep. 2013 Jul 25;4(2):238-47. doi: 10.1016/j.celrep.2013.06.017. Epub 2013 Jul 11. 10.1016/j.celrep.2013.06.017 PubMed 23850288