pSico-shNKCC1
(Plasmid #83027)

• Purpose: expresses an shRNA against NKCC1
• Depositing Lab: Angelique Bordey
• Publication: Young et al J Neurosci. 2012 Sep 26;32(39):13630-8.

Backbone

• Vector backbone: pSico
• Backbone manufacturer: Jacks Lab (MIT)
• Backbone size w/o insert (bp): 7566
• Total vector size (bp): 7609
• Vector type: Mammalian Expression, Lentiviral, RNAi, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: shNKCC1
• Alt name: NKCC1 shRNA
• gRNA/shRNA sequence: ACACACTTGTCCTGGGATT
• Species: M. musculus (mouse)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pSico was obtained from Addgene (plasmid 11578). The NKCC1 shRNA was provided by Drs. D. D. Wang and A. R. Kriegstein, University of California, San Francisco.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a c4351t substitution within the shRNA. The depositing lab states that they do not know whether this mutation affects plasmid function.

pSico allows for conditional (Cre-Lox), stable expression of shNKCC1. Cre expression excises GFP and allows for shNKCC1 expression.