p5E-EF1a/b-globin
(Plasmid
#82582)
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Purpose5' entry vector containing frog translation elongation factor 1 alpha (EF1a) enhancer fused to rabbit b-globin intron for semi-ubiquitous expression
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 82582 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR P4-P1R
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Backbone manufacturerInvitrogen
- Total vector size (bp) 3914
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFrog EF1a enhancer fused to rabbit b-globin intron
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Insert Size (bp)1154
- Promoter None
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was also used in Fowler et al., 2016 (PMID: 27500400) and is part of the associated kit.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p5E-EF1a/b-globin was a gift from Kryn Stankunas (Addgene plasmid # 82582 ; http://n2t.net/addgene:82582 ; RRID:Addgene_82582) -
For your References section:
Spatial and temporal control of transgene expression in zebrafish. Akerberg AA, Stewart S, Stankunas K. PLoS One. 2014 Mar 18;9(3):e92217. doi: 10.1371/journal.pone.0092217. eCollection 2014. PONE-D-13-46380 [pii] PubMed 24643048
Map uploaded by the depositor.
