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pFUSEss-CHIg-mG1_M18
(Plasmid #82357)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 82357 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFUSEss-CHIg-mG1
  • Backbone manufacturer
    Invivogen
  • Backbone size w/o insert (bp) 4492
  • Total vector size (bp) 4829
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Top10 or DH5alpha could be selected on LB LOW SALT with 35-50 micrograms/ml Zeocin.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse immunoglobulin heavy chain IgG1 isotype
  • Alt name
    IgG1 HC
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    367
  • Mutation
    none
  • GenBank ID
  • Entrez Gene
    Ighg1 (a.k.a. IgG1, Igh-4, VH7183)
  • Promoter hEF1-HTLV prom
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AfeI (not destroyed)
  • 5′ sequencing primer GCGAAGGAGGGCCACCATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFUSEss-CHIg-mG1_M18 was a gift from Joanna Bereta (Addgene plasmid # 82357 ; http://n2t.net/addgene:82357 ; RRID:Addgene_82357)

  • For your References section:

    Agglutinating mouse IgG3 compares favourably with IgMs in typing of the blood group B antigen: Functionality and stability studies. Klaus T, Bzowska M, Kulesza M, Kabat AM, Jemiola-Rzeminska M, Czaplicki D, Makuch K, Jucha J, Karabasz A, Bereta J. Sci Rep. 2016 Aug 3;6:30938. doi: 10.1038/srep30938. 10.1038/srep30938 PubMed 27484487
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